Creating a list of clusters

A list of all suprathreshold clusters will be automatically generated when both t-thresholds ("original threshold" and "secondary threshold") are defined. The screen then should look like this:

screenshot02

If you do not know the t-values, you can simply click on [go!] (upper right corner), then MASCOI will calculate them from the specified p-values and the number of degrees of freedom. When loading the t-map, MASCOI obtaines the degrees of freedom from the ASCII comment in the header file (spmT*.hdr). To me this appears a little quick and dirty, but it works. No guarantee, as always...

If the primary ("original") t-threshold is the same as the one used during the SPM analysis, the displayed list (to be more precise, the left part of it) should match the SPM report (it differs in that clusters are ordered only by size and negative clusters are also shown).

MASCOI allows to set a more liberal "secondary" threshold that can be used to create enlarged clusters for illustrative purpose. You can explore your data by setting any threshold, however it is strongly recommended to set a secondary threshold of at least p=0.05.

Please have a look at the rightmost column ("shift [mm]"). It shows how far the corresponding local maxima in the t-map and the contrast map are apart.


Left-Right flipping

The byte order in *.img files varies between labs and SPM versions. MASCOI calls SPM to determine if the images are to be left-right flipped or not. Some uncertainty remains, e.g. when using old data with a new version of SPM. If you are running MASCOI for the first time or if you feel unsure about left and right, compare MASCOI's list (MNI x<0 is left by definition) with an external reference. You can check/change the current settings by clicking on X-Flip at the menubar.